Given below is the TEMPLATE STRAND OF A gene. You are a super enzyme that can do
ID: 134330 • Letter: G
Question
Given below is the TEMPLATE STRAND OF A gene. You are a super enzyme that can do the job of DNA polymerase, RNA polymerase and ribosomes and many other enzymes involved. 1. Synthesize the complementary strand (3) In Eukaryotes can the RNA polymerase bind directly to the DNA? What does it need? 2. 3. Usually what is the consensus promoter binding site. Mark it on DNA 4. Mark the introns. (Introns are in the DNA and spliced in the mRNA. So mark the introns first in the DNA, then transcribe and then splice) Intron 1 is from nucleotides 55---66 Intron 2-is from nucleotides 81--92 Intron 3 is from nucleotides 115-125 5. Start transcription from the 10th nucleotide after the promoter. Include the 10th nucleotide-(3)Explanation / Answer
Ans 1..
Th complementary DNA strand will be from 5' to 3'
Replace A with T
G with C
C with G
T with A
TTCGGATGTA ATCTATATTG GCGGGGTCAAT AGTGCGGGCG TGGATATGGC TCCACCCCTC GTGCTACTAA CTACTGGTTT CCCGGTCTCC CTATCCATCC CATAATTATT GTCAATAGTC TCCTAGCCCA GGCTATGGCA
Ans 2....RNA polymerase in eukaryotic cells require transcription factors to help bind to the promotor region and then the further transcription is carried out
Ans 3...
Usually the promotor region is the TATA box
The DNA sequence for this is TATAAA
Ans 4...intron 1...
In DNA GGGGAGCACGAT
In rna CCCCUCGUGCTA
Intron 2...
In DNA ...GGGCCAGAGGGA
In rna...CCCGGUCUCCCU
Intron 3....
In dna...TATCAGAGGAT
Un rna...AUAGUCUCCUA
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