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Given below is the TEMPLATE STRAND OF A gene. You are a super enzyme that can do

ID: 134330 • Letter: G

Question

Given below is the TEMPLATE STRAND OF A gene. You are a super enzyme that can do the job of DNA polymerase, RNA polymerase and ribosomes and many other enzymes involved. 1. Synthesize the complementary strand (3) In Eukaryotes can the RNA polymerase bind directly to the DNA? What does it need? 2. 3. Usually what is the consensus promoter binding site. Mark it on DNA 4. Mark the introns. (Introns are in the DNA and spliced in the mRNA. So mark the introns first in the DNA, then transcribe and then splice) Intron 1 is from nucleotides 55---66 Intron 2-is from nucleotides 81--92 Intron 3 is from nucleotides 115-125 5. Start transcription from the 10th nucleotide after the promoter. Include the 10th nucleotide-(3)

Explanation / Answer

Ans 1..

Th complementary DNA strand will be from 5' to 3'

Replace A with T

G with C

C with G

T with A

TTCGGATGTA ATCTATATTG GCGGGGTCAAT AGTGCGGGCG TGGATATGGC TCCACCCCTC GTGCTACTAA CTACTGGTTT CCCGGTCTCC CTATCCATCC CATAATTATT GTCAATAGTC TCCTAGCCCA GGCTATGGCA

Ans 2....RNA polymerase in eukaryotic cells require transcription factors to help bind to the promotor region and then the further transcription is carried out

Ans 3...

Usually the promotor region is the TATA box

The DNA sequence for this is TATAAA

Ans 4...intron 1...

In DNA GGGGAGCACGAT

In rna CCCCUCGUGCTA

Intron 2...

In DNA ...GGGCCAGAGGGA

In rna...CCCGGUCUCCCU

Intron 3....

In dna...TATCAGAGGAT

Un rna...AUAGUCUCCUA

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