Given below is the TEMPLATE STRAND OF A gene. You are a super enzyme that can do

Question

Given below is the TEMPLATE STRAND OF A gene. You are a super enzyme that can do the job of DNA polymerase, RNA polymerase and ribosomes and many other enzymes involved. 1. Synthesize the complementary strand (3) In Eukaryotes can the RNA polymerase bind directly to the DNA? What does it need? 2. 3. Usually what is the consensus promoter binding site. Mark it on DNA 4. Mark the introns. (Introns are in the DNA and spliced in the mRNA. So mark the introns first in the DNA, then transcribe and then splice) Intron 1 is from nucleotides 55---66 Intron 2-is from nucleotides 81--92 Intron 3 is from nucleotides 115-125 5. Start transcription from the 10th nucleotide after the promoter. Include the 10th nucleotide-(3)

Explanation / Answer

1) Complementary strand runs in the opposite direction of the template strand. The nucleotide bases bind with each other according to the base pairing rule. Adenine binds with rhyminecthrough two hydrogen bonds while guanine binds with cytosine through three hydrogen bonds. The complementary strand of the given template strand is given below,

5' TTCGGATGTA ATCTATATTT GCGGGTCAAT AGTGCGGGCG TGGATATGGC TCCACCCCTC CTTATTGTCA GTGCTACTAA CTACTGGTTT CTATCCATCC CATAATTATT GTCAATAGTC TCCTAGCCCT GGCTATGGCA 3'

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