. Outline the steps involved in an endospore stain (remember smear prep is a par
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. Outline the steps involved in an endospore stain (remember smear prep is a part of or the Gram stain, what reagents act as the primary which reagents act as the primary stain and counterstain. Understand why endospores ar 8. Why is a dilution series often done before making colony counts? (think along the ines of number why endospores are diffcuit to stain organisms and what you would see on the plate) 9. Be able to recognize a proper streak for isolatio 10. What is the Kirby-Bauer Disk Diffusion Method? What is meant by a "Zone of r plate, be able to measure this. For what reason(s) migh antimicrobial show no zone of inhibition? n and know the purpose of performing this procedure t a chemical that is usually thought to be a good 11. How would you test for the ability of a microbe to produce catalase? 12. What medium is used to test for hemolysis? Why is this an important test in a clinical lab? 13. Be able to identify the following on microscope slides: Gram positive bacteria, Gram negative bactenia. the three basic shapes of bacteria, endospores, negative stain amecium, sample is viewed to " Handout to view these organisms OR you 14. Be able to identify the following on microscope slides: Aspergillus, Penicillium, Euglena, Oscillatoria, Trypanosoma. For the last genus in this list, what type diagnose the infection? Use the "Survey of Microorganisms can view the actual slides on a microscope in the ACE Center (SSB 2201). 15. Know what bacteriophage and viral plaques are; Know howl/why they are related:, Understand why bacteriophage must be grown in bacteria 16. Understand what a plasmid is, and be able to briefly explain how it can be inserted into a bacterium. Know what this process is called. 17. Why can't bacteria be stained using acidic dyes? What are basic dyes? Be able to explain the general concept in terms of the use of these types of dyes on bacteria. Yes we discussed this! 18. Understand how UV light affects bacterial cells and bacterial endospores. 19. Understand the significance of biochemical tests in the identfication of microorganisms 20. Be able to identify an organism using a dichotomous key or a version of the chart you have been using to determine your unknown organisms. 21. Be familiar with the GENERAL lab safety rules. If you follow them, then this should be easy letures posted on CanvasExplanation / Answer
Answer 8
Answer 9
streak plate technique is used for the isolation into pure culture of the organisms (mostly bacteria), from mixed population. The inoculum is streaked over the agar surface in such a way that it “thins out” the bacteria. Some individual bacterial cells are separated and well spaced from each other. As the original sample is diluted by streaking it over successive quadrants, the number of organisms decreases. Usually by the third or fourth quadrant only a few organisms are transferred which will give discrete colony forming units (CFUs).
Answer 10
Kirby-Bauer Test, Is a test of the antibiotic sensitivity of bacteria , A Zone of Inhibition Test, also called a Kirby-Bauer Test, is a qualitative method used clinically to measure antibiotic resistance and industrially to test the ability of solids and textiles to inhibit microbial growth.
Answer 11
Catalase-negative bacteria do not respire using oxygen as a terminal electron acceptor and may be facultative anaerobes that only ferment and
Answer 12
A nutrient medium augmented with the addition of 5% sheep blood routinely is used. Such a medium is called blood agar. Hemolysis is determined by streaking for isolation on a blood agar plate.
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