Part A Complete the outline of an experiment, which determines if eukaryotic tra

Question

Part A Complete the outline of an experiment, which determines if eukaryotic transcription factors (TF) bind to the fragment Assume all necessary reagents, equpn experimental know-how are available in the laboratory Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Not all terms will be used. Reset Help bind higher thus do not bind control sample present lower For the band shift assay, two samples of the DNA fragment are analyzed by agarose gel electrophoresis one sample is mixed with TFs without the TFs subjected to gel electrophoresis and the relative mobility of the two DNA samples is compared if TFs are ), and one is treated identically except ) After allowing sufficient time for TFs to bind to DNA, the samples are and to the DNA, the experimental sample wil have a electrophoretic mobility than the control sample absent experimental sample Submit RequeatAnswer Part B Complete previous part(s)

Explanation / Answer

FOR THE BAND SHIFT ASSAY , TWO SAMPLES OF DNA FRAGMENT ARE ANALYSED BT AGROSE GEL ELECTROPHORESIS ONE SAMPLE IS MIXED WITH TFs EXPERIMENTAL SAMPLE AND ONE IS IDENTICALLY TREATED EXCEPT WITHOUT TFs CONTROL SAMPLE AFTER ALLOWING SUFFICIENT TIME FOR TFs TO BIND TO DNA THE SAMPLES ARE SUBJECTED TO GEL ELECTROPHORESIS AND THE RELATIVE MOBILITY OF THE TWO DNA SAMPLES IS COMPARED . IF TFs ARE PRESENT AND BIND TO THE DNA . THE EXPERIMENTAL SAMPLE WILL HAVE A LOWER ELECTROPHORESIS MOBILITY THAN THE CONTROL SAAMPLE

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