Yellow light of wavelength 570 nm is used to view an object under a microscope.
ID: 1402904 • Letter: Y
Question
Yellow light of wavelength 570 nm is used to view an object under a microscope. The objective lens diameter is 7.55 mm.
(a) What is the limiting angle of resolution?
µrad
(b) Suppose it is possible to use visible light of any wavelength. What color should you choose to give the smallest possible angle of resolution, and what is this angle?
(c) Suppose water fills the space between the object and the objective. What effect does this change have on the resolving power when 570-nm light is used?
Explanation / Answer
a)
here by using the formula
theta(min) = 1.22 (lambda / D)
theta(min) = 1.22 (( 570 * 10^-9) / (7.55 * 10^-3))
theta(min) = 92.1 * 10^-6 rad
b)
smallest visible wavelength(violet) at 400 nm
theta(min) = (1.22 * 400 * 10^-9 )/ (7.55 * 10^-3)
theta(min) = 64.6 urad
c)
when water fills the space
theta(min) = 1.22 * lambda / D * n(water)
theta(min) = 1.22 * 570 * 10^-9 / (7.55 * 10^-3 * 1.33)
theta(min) = 69.2 urad
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