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a to k he cell auickly by denaturing f cel b. to care the cells adhert ho phe sh

ID: 141604 • Letter: A

Question

a to k he cell auickly by denaturing f cel b. to care the cells adhert ho phe shide more re 1. What two purposes does fixing the cell serve? For cach problem listed below, choose ONE reason it might occur and ONE solution. Reasons and solutions may be used more than once. 2. One cannot find any cells on the slide. REASON One cannot tell the spatial arrangement because the cells are layered one on top of each a. SOLUTION b. other REASON One has determined that the cells are distorted. REASON SOLUTION c. SOLUTION

Explanation / Answer

2a) REASON -There may be many reasons for this to happen -

SOLUTION - The observer should be very keen about the procedure of preparing the slide . He should know about the microscope well to avoid any focussing defects .

2b) REASON - During the preparation of the slide , multilayered slide may have been prepared instead of a single layer . This may cause the problem

SOLUTION - There should be a single cell layered slide preparation to avoid the problem .

2c) REASON - During the heat fixation step of the slide preparation , the slide may be overheated .

SOLUTION - Heat fixation should be done correctly and should avoid over heating the cells .