B5171 You carry out the following serial dilution and plate 50 ul of the Answer
ID: 142750 • Letter: B
Question
B5171 You carry out the following serial dilution and plate 50 ul of the Answer the hefolowing oung bonal dhuton and late S u of the nal reuingh 42 co work to receive full credit Cel following questions. You must include units and show your should be reported as cells/ml euture 42 colonies (1.5 pts) What is the concentration of cells in Tube c? 5. (1.5 pts) What is the concentration of cells in tube b? (1 5 pts) What is the concentration of ceils in tube a? (1.5 pts) What is the concentration of cells in the original culture tube?Explanation / Answer
4) In the tube C, 42 colonies have formed from 50 ul. So, the concentration in tube C is,
= (1000 × 42)/50
= 42 × 20
= 840 colonies/ml
5) In tube B, 100 ul contains 420 colonies. So, the concentration in tube B is,
= (1000 × 420)/100
= 4200 colonies/ml
6) In tube A, 50 ul contains 2100 colonies. So, the concentration of bacteria in tube A is,
= (2100 × 1000)/ 50
= 42000 colonies/ml
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