This is all the information provided. It\'s a fictional gene they are talking ab

Question

This is all the information provided. It's a fictional gene they are talking about in the problem but in class we are discussing in class is LDH-A

The website mentioned in the problem is: https://www.thermofisher.com/us/en/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/molecular-cloning/cloning/traditional-cloning-basics.html

5. Experimental design: You have been given a plasmid with your gene of interest (YFG, Your Favorite Gene) called "won't_work_YFG". This plasmid does not have the capacity to express proteins, which you need to do. In your lab you also have an expression plasmid that does not have your gene of interest in it (NMG, Not My Gene) called "works_NMG". Your job, as an undergraduate student, is procedure to make "works_YFG" from the two previously mentioned plasmids. Design and describe that procedure here. You may find this website useful in helping you with this design. The best answer to this question wil include the design and also a way to verify that your design worked. (16 points)

Explanation / Answer

The gene, "works YFG", can be made from two genes "won't work YFG" and "works NMG" in the following way,

The success of the procedure can be verified by performing any one of the following,

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