This is all the information i was given. Please help me in anyway possible with

Question

This is all the information i was given. Please help me in anyway possible with the chart!

5) You are studying the ability of your cultured cells to increase Ca== intracellularly in response to a newly identified agent you have extracted from a soil fungus during a pharmaceutical screen. You are trying to identify HOW that increase in [ Ca==] is occurring, ie via entry of Ca from extracellular medium OR the ability of this novel agent to elevate intracellular [ Ca==] via activation of IP3 production. Outline THREE DIFFERENT approaches you would use, and the outcomes you would expect IF the increase in intracellular [ Ca==] was from extracellular sources or release of Ca== from intracellular stores.

Use the format below to describe your experimental strategy, and then the expected outcomes ( i.e., IF…..THEN…. ) Experimental Strategy IF Ca++ from EXTRACELLULAR STORES If Ca++ from INTRACELLULAR IP3-responsive stores Use the format below to describe your experimental strategy, and then the expected outcomes ( i.e., IF…..THEN…. )

Experimental Strategy IF Ca++ from EXTRACELLULAR STORES If Ca++ from INTRACELLULAR IP3-responsive stores 1) 2) 3.)

Explanation / Answer

IP3 acts as central mediator of calcium mobilization. It acts on endoplasmic reticulum and binds to IP3 receptor. The IP3 receptor acts as calcium channel and allows release of calcium from the endoplasmic reticulum.

Thus, the decrease in calcium concentration in endoplasmic reticulum initiates the movement of calcium extracellular into the cell. The continuous movement of calcium from extracellular stores results in depletion of ATP levels. This is because the energy released by hydrolysis of ATP is used for movement of calcium from extracellular store to cell interior.

During movement of calcium from cell to exterior, the sodium is transported into the cell. Thus, sodium and calcium movement is coupled.

The decrease of extracellular calcium level results in hypocalcemic tetany. In severe hypocalcemia, blood coagulation and signal transduction are affected. The high concentration of calcium in the cell may result in apoptosis or death by necrosis.

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