1) A) Why are cells thawed quickly rather than slowly? B) What is the function o

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1) A) Why are cells thawed quickly rather than slowly? B) What is the function of trypsin in a dissociation media? And why is EDTA added to the trypsin? C) if a pellet of HeLa cells was suspended in 4.2 mL DMEM+, how many mL of this suspension would you transfer into a new flask to perform a 1:6 split? [show calculations) part/whole =part/whole
1) A) Why are cells thawed quickly rather than slowly? B) What is the function of trypsin in a dissociation media? And why is EDTA added to the trypsin? C) if a pellet of HeLa cells was suspended in 4.2 mL DMEM+, how many mL of this suspension would you transfer into a new flask to perform a 1:6 split? [show calculations) part/whole =part/whole
B) What is the function of trypsin in a dissociation media? And why is EDTA added to the trypsin? C) if a pellet of HeLa cells was suspended in 4.2 mL DMEM+, how many mL of this suspension would you transfer into a new flask to perform a 1:6 split? [show calculations) part/whole =part/whole

Explanation / Answer

A cells are quickly to reduce exposure to damaging ice crystals when the vial is partially thawed

Ans B trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured. When added to a cell culture, trypsin breaks down the proteins which enable the cells to adhere to the vessel. EDTA a chelating agent is added to enhance enzymatic activity offtrypsin solution

Ans C

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