Suppose you want to identify the bacterial species present in a polluted stream

Question

Suppose you want to identify the bacterial species present in a polluted stream and in a clean stream Scientists have traditionally identified bacterial species by comparing the physical and biochemical properties of laboratory-grown cultures. However, yau cannot grow many of these bacteria in culture. How can you identify the species from the two streams without culturing the bacteria in the laboratory? Measure the pH and chemical attributes of each stream to hypothesize what types of bacteria can exist in these environments. Find organisms in the stream that the bacteria can infect and use these hosts to keep Transform a common laboratory strain of Escherichia col with DNA isolated from the the bacteria alive during laboratory analysis. O unknown bacteria, then measure the physical and biochemical properties of the transformed E. col Detect bacterial species by sequencing DNA isolated from water samples collected at each stream.

Explanation / Answer

1. Option is 4. We can easily detect the bacterial species by sequencing the DNA isolated from the two water samples at each stream.

2. The genotype is raf +

Explaintion.. raffinose is the sugar molecule composed of glucose galactose and fructose. Of course it is very rich in the carbon sugar so for the growth of the bacteria the colony can utilize the carbon source from the raf.

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