3 27) In E coli, which direction? A) primase B) DNA ligase C) DNA polymerase III
ID: 143938 • Letter: 3
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3 27) In E coli, which direction? A) primase B) DNA ligase C) DNA polymerase III D) helicase enzyme catalyzes the elongation of a new DNA strand in the S t a specific area of a chromosome, the sequence of nucleotides below is present where the chain opens to form a replication fork: An RNA primer is formed starting at the underlined T CD of the template. Which of the following represents the primer sequence? A) 5' GCCTAGG3 B)5'ACGTTAGG 3 C)5, A C G U U A G G 3. D)5, G C C U A G G 3. 29) In E. coli, what is the function of DNA polymerase II1? A) to unwind the DNA helix during replication B) to seal together the broken ends of DNA strands C) to add nucleotides to the 3' end of a growing DNA strand D) to degrade damaged DNA molecules 30) Which of the following statements correctly describes the difference between the leading and the lagging strands of DNA during DNA replication? fork, and the lagging strand is synthesized in the opposite direction. B) The leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5' end. C) The lagging strand is synthesized continuously, whereas the leading strand is synthesized in short fragments that are ultimately stitched together. D) The leading strand is synthesized at twice the rate of the lagging strand. 31) In his work with pneumonia-causing bacteria and mice, Griffith found that A) the protein coat from pathogenic cells was able to transform nonpathogenic cells. B) heat-killed pathogenic cells caused pneumonia. C) some substance from pathogenic cells was transferred to nonpathogenic cells, making them pathogenic. D) the polysaccharide coat of bacteria caused pneumonia.Explanation / Answer
Ans 27. Option C.
DNA polymerase III catalyse the synthesis of new DNA strand in 5' to 3' direction.
In E.coli pol III is the major replicating enzyme that catalyzes the synthesis of both leading and lagging strand synthesis.
28. Option C.
The RNA primer will be 5' ACGUUAGG 3' because the primer we take should be of opposite polarity. We take U base against A and G base against C and vice versa.
29. Option C.
DNA polymerase add nucleotides to the 3' end of the growing polypeptide chain.
DNA pol III is a huge multiprotein enzyme complex that consist of 10 distinct polypeptides.
30. Option A.
The leading stand is synthesized in the same direction as the movement of replication fork and lagging strand is synthesized in the opposite direction.
The DNA ligase enzyme is used for the joining of okazaki fragments in the lagging strand synthesis.
31.option D.
The polysaccharide coat of the bacteria caused the pneumonia.
He used the two strains of bacteria one is S strain and other is R strain. The S strain synthesize the polysaccharide capsule that protect it from the host immune system and cause the death of the host however R strain do not synthesize polysaccharide capsule and hence defeat by the host immune system.
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