3. In order to see both acid-f two different species of bacteria. However, we on

Question

3. In order to see both acid-f two different species of bacteria. However, we only used 1 species of bacteria to make the endospore slide and we were able to see tw fast and non-acrd-fast cells on the same slide, it was necessary to use o different types of cells. Explain this. 4. You perform an endospore stain on an unknown culture and only observe pink cells. Can you conclude definitively that this is not an endospore-forming bacteria? Explain. 5. The Mycobaterium cultures we used for the acid-fast stain and the Bacillus cultures we used for the endospore stain were both roughly 72 hours old. Why do we need to use older cultures to be successful with these stains? (There is a different reason for each bacteria). 131

Explanation / Answer

3) In endospore staining only one bacterial species is used. Among the bacteria, only few form endospore. These endospores are acid fast and they retain the primary stain during acid fast staining. Those bacterial cells that have not formed endospores are non acid fast and they take up the secondary stain. So, we can observe both acid fast and non acid fast cells in single species of bacteria.

4) Acid fast cells are stained pink and non acid fast cells are stained blue. So, in endospore staining, if all the cells appear pink, all took up the primary stain.

5) The endospore staining is done on 72 hours old specimen to allow sufficient time for the formation of an endospore.

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