if what an promoter element? What does the minimal initiation complex consist of
ID: 144218 • Letter: I
Question
if what an promoter element? What does the minimal initiation complex consist of? What is an enhancer sequence and where could it be located? What is a protein bridge attached to - that is, what does it bridge? Determining and verifying functionally significant components of a promoter (or other functionally significant region of DNA that binds proteins): 17. Consensus sequence analysis (Genetic Analysis 8.1 and ppt slide 20). If a base is invariant in sequences from many different species what does this mean? If a base shows all four possible nucleotides in that same comparison what does that mean? 18. Band shift analysis - if the position of a band of DNA does not shift its position in the gel what does this mean? If the position of the band indicated that it is significantly larger than before the test what does this mean? 19. DNA Footprint Protection Assay -what does the word protection mean in this context? What molecule is being protected from which enzyme? What do we look for on a gel using this technique? 20. Mutation analyses to determine which bases are most functionally significant examine transcription level when a single base is mutated. This is done for a number of bases in the promoter region. In bacterial DNA where are the bases located that when mutated strongly decrease level of transcription?Explanation / Answer
17. Consensus sequence analysis:
DNA (or RNA) base sequences + Amino acid base sequences.
18. Band shift analysis:
19. DNA foot printing protection assay:
20. Mutation analysis:
a. Structural genes b. Controlling genes- promoter/ operator.
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