10) List two advantages and two disadvantages of CRISPR Cas-9 treatment versus c

Question

10) List two advantages and two disadvantages of CRISPR Cas-9 treatment versus conventional gene therapy. 11) At pH 6, the total charge of a lysine amino acid is closest to: (circle one choice) -2 -1 +1 +2 +3 12) Draw the chemical structure of the peptide Asp-Lys-Cys-His-Ser-Thr-Tyr, as it would be in aqueous solution at pH 7. Label the peptide bonds. What is the (approximate, nearest integer) total charge of this peptide at pH 7? Which functional groups are charged at PH 7? a) What is the (approximate, nearest integer) total charge of this peptide at pH 1.5? Which functional groups are charged at pH 1.57 b) What is the (approximate, nearest integer) total charge of this peptide at pH 12? Which functional groups are charged at pH 12 13) Which of these amino acid side chains can form hydrogen bonds with each other? A) Ala and Pro D) Asn and Ala B) Phe and Tyr E) His and Ala C) Tyr and Gin F) His and Ser 14) Rank the following amino acids according to their solubility in water: Serine, phenylalanine, tyrosine. Explain your reasoning.

Explanation / Answer

10.

Gene therapy is a broad strategy for curing disease by modifying, supplying or blocking genes or gene products that cause a condition either by their presence or absence. The treatments typically involve delivery of synthetically manufactured nucleic acid polymers, which are relatively expensive to manufacture. Early tries caused unintended outcomes that suppressed progress.

CRISPR cas-9 is a relatively new DNA editing technique that has advantages like : it allows low cost, precisely-targeted single base modifications as well as It has the potential to revolutionize how gene therapy is implemented, however it has disadvantages as by its terribly nature is making major moral issues.

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