Use Swiss PDBViewer (or your favorite structure viewer) to analyze the structure
ID: 144444 • Letter: U
Question
Use Swiss PDBViewer (or your favorite structure viewer) to analyze the structures and answer the following questions.
Examine the 2 pairs of proteins below. In each case, these are enzymes that catalyze the same reaction in different species. The question is: are they related or not? i.e. Are the structures essentially the same? Often you can get similar reactions from proteins that evolved from a common ancestor (e.g. trypsin and chymotrypsin). Sometimes the same reaction can be catalyzed by proteins with very different evolutionary origins and with very different structures (e.g. trypsin and subtilisin). Your job is to decide if these proteins have fundamentally different folds or if they have basically the same fold. Briefly justify your answer (3 sentences).
1. Aldolase from an Archaea and a Bacillus (PR1A & PR1B)
2. Isochorismate synthase from a Proteobacterium and a Bacillus (PR2A & PR2B)
Explanation / Answer
There were two classes of aldolase for catalysis of glyceraldehyde 3- phosphate .. recently the genes encoding for this was found in archea, although they do not share the significant features of the sequence with the traditional members of aldolases .. Thermoproteus tenax, a member of Archean group , in which there is a significant conservation of six catalytic - substrate binding residues .. this serves as the main difference between the classic aldolase and archeal aldolase ..
2. Isochorismate synthase is an catalytic enzyme that converts chorismate into isochorismate . Bacellus has a duplicate genes of this enzyme .. prominent use of this enzyme is biosynthesis of respiratory component menaquinone and the siderophore 2-3 dihydroxybenzoate..
Where in the proteobacterium,two different isoenzymes involved for the process .. MenF and EncT ..
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