6. nosepiece 2. If the ocular lens on your microscope magnifhed 8x rather than t
ID: 144705 • Letter: 6
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6. nosepiece 2. If the ocular lens on your microscope magnifhed 8x rather than the typical 10x what would be the final magnification for each objective lens? 1. Low: 2. High: 3. Oil Immersion: 3. How does the oil act upon the light so you may better visualize bacteria at 1000x magnification? 4. What is the meaning of partocal and what is the advantage of parfocal lenses? 5. Refer to Part V of your lab: 1. Before you focus a specimen with the oil immersion lens with which other lenses ün order) should you have fist resolved the specimen 2. With which lensles) are you permitted to use both coarse and fine focus? 3. With which lenses should you only use fine focus? Text Entry Copy and paste or type your submission right here Diaphragm- controls the amount of light hitting the specimen. Ocular lens- used to view the object under the lens Nosepiece-Hoids two or more objec be rotated to change the power 1 Condenser- To focus the light on the specimenExplanation / Answer
Hi
2. If the ocular lens is magnifed at 8x, then the magnification for each objective lens will be. 1) Low: magnification will reduce
2) High: magnification reduction will not be significant
3) oil immersion: no change as the magnification using oil can go as high as 1000x, small change of 8x will not be counted.
3. When the light rays pass from one object to other , say from air into lens or glass, they bend by certain angle. this bending reduces or take away light from the lens. When we use high magnification like 100x, much of the light from air into glass is lost by bending. This is because the refractive index of glass is 1.5 and that of air in 1. So if we use a medium with same refractive index as glass, light does not bend while passing throght the mediums, so oil is used which also has same refractive index as glass. hence image clarity increases.
4. Parfocal lens does not loose focus when we zoom in or zoom out. These lenses stay focued when we magnify or change the object piece during microscopy. This helps to focus on tiny mirobial morphologies and highlight them. If focus is lost during magnification the visuals may change and so may be the information.
5. 1) before oil immersion we must first resolve images in 10x followed by 40x and then onto 100x
2). in 40x we can use both coarse and fine focus
3) when using 100 oil immersion, only fine focus must be used.
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