When considering anion exchange chromatography, which of the following statement
ID: 145435 • Letter: W
Question
When considering anion exchange chromatography, which of the following statements best describes how increasing concentrations of NaCl elute the protein from the column?
Option A: The proteins that are bound to the chromatography resin are exchanged for Cl–.
Option B: The Na+ ions that are bound to the chromatography resin are exchanged for proteins.
Option C: The proteins that are bound to the chromatography resin are exchanged for Na+.
Option D: The Cl– ions that are bound to the chromatography resin are exchanged for proteins.
Given your answer to the question above, which of the following methods would be best suited for a one-step purification of lactate dehydrogenase from a hypothetical mixture of the three proteins?
Option A: anion exchange chromatography using a pH 9 buffer
Option B: anion exchange chromatography using a pH 5 buffer
Option C: anion exchange chromatography using a pH 10.5 buffer
Option D: anion exchange chromatography using a pH 7 buffer
Which of the following combinations of methods would be best suited for the purification of cytochrome c from a hypothetical mixture of the three proteins?
Option A: Gel filtration chromatography to obtain the low molecular weight proteins, followed by cation exchange chromatography at pH 8.
Option B: Cation exchange chromatography at pH 10, followed by elution at high [NaCl], and final purification using gel filtration chromatography.
Option C: Anion exchange chromatography at pH 10, followed by elution at high [NaCl], and final purification using gel filtration chromatography.
Option D: Cation exchange chromatography at pH 7, followed by elution at high [NaCl], and final purification using gel filtration chromatography.
How many amino acids are in the longest form of the human DNA methyltransferase?
Option A: 1511
Option B: 1882
Option C: 1606
Option D 1678
Explanation / Answer
When considering anion exchange chromatography, which of the following statements best describes how increasing concentrations of NaCl elute the protein from the column?
Anion-exchange chromatography is a process that seperates substances based on their charges using resins conatining positive groups that are exchanged.
Hence, the correct answer is
Option B: The Na+ ions that are bound to the chromatography resin are exchanged for proteins
Given your answer to the question above, which of the following methods would be best suited for a one-step purification of lactate dehydrogenase from a hypothetical mixture of the three proteins?
Lactate Dehydrogenase can be purified by anion exchange chromatography at near pH 7 as it is an ideal pH for its purification.
So, a buffer with pH near 7 should be used.
Hence, the correct answer is
Option D: anion exchange chromatography using a pH 7 buffer
Which of the following combinations of methods would be best suited for the purification of cytochrome c from a hypothetical mixture of the three proteins?
Purification of cytochrome c requires cation excahnge chromatography at pH 7.
Hence, the correct answer is-
Option D: Cation exchange chromatography at pH 7, followed by elution at high [NaCl], and final purification using gel filtration chromatography.
How many amino acids are in the longest form of the human DNA methyltransferase?
Option A: 1511
1511 amino acids are conatined in the enzyme.
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