ave ea Tyr or a Trp residue as it absorbs 280 nm. C. It has two Tyr residues. D.

Question

ave ea Tyr or a Trp residue as it absorbs 280 nm. C. It has two Tyr residues. D. It has a Trp residue at its C-terminus. E. It has a Trp residue at its N-terminus. 9. Insulin was analyzed via SDS PAGE, predict which reagents may have been added to lanes 4 and 5 A. B-mercaptoethanol was added to lane 5 only B. -mercaptoethanol was added to both lanes 4 and 5. C. -mercaptoethanol was added to lane 4 only. D. Lane 5 is a mixture of periproinsulin and insulin, and lane 4 is periproinsulin E. Lane 5 is a mixture of signaling peptide and insulin, and lane is the proinsulin. 10. Which lane on the gel is likely to correspond to periproinsulin? Lin Lane # 1 2 3 A. 1 B. 2 D. 4 E. 5 45-

Explanation / Answer

I believe the correct answer to be:

9) A) B mercaptoethanol is added to lane 5 only since this reagent will remove disulfide bond dividing the insulin into 2 different sized peptide.

10) C) 3

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