Please show all the steps :- 1- A culture was diluted by adding a 10L aliquot to

Question

Please show all the steps :-

1- A culture was diluted by adding a 10L aliquot to 990L water. Then, 10L of this dilution was plated out, yielding 67 colonies. Calculate the CFU/mL in the original culture:

6.7 x 105 cfu/ mL

6.7 x 104 cfu/mL

6.7 x 103 cfu/mL

6.7 x 102 cfu/mL

2- A 100 l sample containing 10 bacterial cells/l in log phase growth is added to 9.9 ml of fresh culture medium broth. Assuming no nutrients are in limited supply, and a generation time of 30 minutes, how many bacteria will be present in the entire broth culture after five hours?

6.0 × 106

2.56 × 107

1.024 × 106

1.2 × 106

6.0 × 105

3- Starting with 10 bacterial cells per milliliter in a sufficient amount of complete culture medium with a 1-hour lag phase and a 30-minute generation time, how many cells will there be in a liter of medium at the end of 2 hours?

4- Suppose you perform a serial dilution of 0.1-ml sample from a liter of culture medium. The 103 plate gives 440 colonies, and the 104 plate gives 45 colonies. Calculate the approximate number of bacteria in the original liter.

Explanation / Answer

Cfu is a unit to measure the amount of viable colony of bacteria. here viable means the ability to divide or to form colonies.

Cfu/ml value in original culture is -6.7 ×105 cfu/ml.

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