9. Gel electrophoresis. The total mass of a protein containing quaternary struct

Question

9. Gel electrophoresis. The total mass of a protein containing quaternary structure was measured by centrifugation, using the technique of sedimentation equilibrium. Under physiological conditions its total mass is 140,000 g/mol The same protein complex was analyzed by SDS-PAGE in the absence or presence of the reducing agent p mercaptoethanol (BME); see the results in lanes A and B in the figure below.(Note that lane C shows a standard of known protein masses.) A7,D 1o (zq.oe%) From these results, describe the native protein complex, in terms of the kinds of subunits present, the stoichiometry of the subunits, and the kinds of bonding (covalent, non-covalent) ex isting between the subunits

Explanation / Answer

Answer: Lane A contains single band, on comparing this band with standard it has been concluded that it is serum albumin protein. It has calcium binding sites. Hydrogen bonds, carbonyl-amide bonds and peptide bonds are presents.

In lane B first band is of ovalbumin and second is of carbonic anhydrase. Bonds present in ovalbumin are peptide bonds and it is globular protein.

Carbonic anhydrase have Zn2+ ion that attaches at the four coordinates. Strong hydrogen bonds are present in active sites.

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