10. How many ul of 20ng/ul stock of DNA do you need to get 60ng of DNA 11. How m

Question

10. How many ul of 20ng/ul stock of DNA do you need to get 60ng of DNA
11. How many ul of a 20ug/ml stock of DNA so you need to get 40ng of DNA
12. Prepare 100 ml of a 10mM tris solution using 1M of tris (include the amount of water in your answer)
13. Prepare 10ml of a 50mM tris solution using 1M of tris (include the amount of water in your answer)
14. Prepare 100ml of a solution that is both 25mM tris and 150mM NaCl using 1M tris stock and 50M NaCl stock
15. Prepare 500 ml of a solution that is 50mM tris, 150mM NaCl, and 1mM EDTA using the following stock: 1M tris, 5M NaCl, 0.5 EDTA

You are having a party for 60 people and you want each person to get an apple. The store sells apples in bags of 20. How many bags of apples do you need to purchase? 9. 10 How many 1 of a 20ngful stock of DNA do you need to get 60ng of DNA? (Hint: compare this question to question 7) 11 . How many 1 of a 20kg/ml stock of DNA do you need to get 40ng of DNA? 12. Prepare 100ml of a 10mM Tris solution using a 1M Tris stock (include the amount of water in your answer) 13. Prepare 10ml of a 50mM Tris solution using a 1M Tris stock (include the amount of water in your answer). 14. Prepare 100 ml of a solution that is both 25mM Tris and 150mM NaCI using a 1M Tris stock and a 5M NaCi stock. (Hint use two separate CrV,equations to calculate the volumes needed of each stock solution and add water to reach the final volume) 15. Prepare 500ml of a solution that is 50mM Tris, 150mM NaCI, and 1mM EDTA using the following stocks: 1M Tris, 5M NaCi, 0.5M EDTA

Explanation / Answer

Please find the answers below:

Answer 10: According to the information, the stock DNA is 20ng/ul. In order to make 60 ng of DNA, a total of 60/20 = 3 ul of this stock is required.

Answer 11: The calculation can be made according to the formula below:

20 * 103 ul = 40 * 10-3 * Volume required

Thus, volume required = (20 * 103) / 40 *10-3 or 5*105 ul

Answer 12: To prepare 100 ml of a 10mM tris solution using 1M of tris, following formula will be used:

100 * 10-3 * 100 = 1 * Volume of water

Volume of water required = 10-3 ml. This means that a total of 0.001 ml of this stock solution must be diluted with water to make up 100 ml of solution. Thus, the volume of water required is 100- 0.001 or 99.999 ml.

Answer 13: To prepare 10ml of a 50mM tris solution using 1M of tris, following formula will be used:

10 * 50 * 10-3 = 1 * volume required

Volume required = 0.5 ml.

Thus, 0.5 ml of the stock solution must be mixed with 9.5 ml of water to get desired dilution.

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