3. The experiment below provides evidence that the e-subunit of bacterial DNA Po

Question

3. The experiment below provides evidence that the e-subunit of bacterial DNA Pol Ill contains the 2 2 7 proofreading activity of the holoenzyme. DNA Pol llI holoenzyme and the purified were incubated separately with the two labeled DNA substrates 3H-label at 3' end of DNA substrates Pol III E-subunit 4 Perfectly paired DNA substrate Perfectly paired DNA substrate 2 5 -T T T T T T3H -3 DNA substrate with mismatches -subunit Mismatched DNA substrate 3 Pol III 5 -T T T T T C3H-3 0.6 Time (minutes) What enzymatic activity does the e-subunit possess that allows for proofreading of DNA during synthesis? 1 point. 5 Explain how the data in the plot below shows that the -subunit has this enzymatic activity: Describe the two DNA substrates used in the experiment. 1 point. Compare the results of the assay using the two DNA substrates - what does the plot show? 2 points

Explanation / Answer

ANS 1

ANS 2 AND 3

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