Describe the ways in which DNA can vary from its ideal B structure, and contrast

Question

Describe the ways in which DNA can vary from its ideal B structure, and contrast the B form of DNA with the A and Z forms. What factors or conditions favor these deviations from the B form and the choice between the three possible forms? 2. While working at the bench, you forgot to label two tubes of DNA strands. You know the sequences of each DNA strand and that they contain different amounts of G:C base pairs, but you do not know which strand is in which tube. Describe how you can use spectrophotometry to properly label your two tubes. 3. 4. Describe the structural features that differentiate RNA from DNA.

Explanation / Answer

3. The ideal structure of DNA is the B structure . It is one of the three biological active form of the DNA

It has right handed turned and is the typical double helical structure . The structures of DNA was given by Watson and Crick.

When the samples of DNA is dehydrated , the B DNA changes its structure to form the A DNA . The angle of inclination is more in the A DNA .

When the structure of DNA is turned left handed, we call it Z DNA.

2.Spectrophotometer is a device which quantitate the nucleic acids .

It counts the average concentration of DNA or RNA in a mixture and also its purity.

So, the more concentrated mixture will have higher optical density , and will reflect less light on the photodetector.

So if we know which sample has more concentration , we can label the two strands.

5.

2.It has single stranded structure.

DNA RNA 1. It has deoxyribose sugar 1. It has ribose 2.It has two stranded

2.It has single stranded structure.

3. It has thymine,adenine , cytosine, guanine as bases . 3.It has uracil in place of thymine.

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