What would you use to join two DNA fragments to each other? Select one: a. Restr

Question

What would you use to join two DNA fragments to each other?

Select one:

a. Restriction enzyme

b. Ligase

c. Chaperonin

d. Plasmid

The activity of pyruvate kinase is controlled by which of the below agents

Select one or more:

a. Fructose-6-phosphate

b. Fructose-1,6-bisphosphate

c. Protein Kinase A

Why do plasmids used to carry foreign genes into E. coli cells invariably also carry the gene for resistance to an antibiotic?

Select one or more:

a. The gene is required for cellular replication

b. The gene is necessary for the lac operon system to operate

c. The gene is necessary to safely grow the bacterium in the laboratory without risk of containment breach

d. To enable selection for only those cells that carry the plasmid with the foreign gene of interest

Explanation / Answer

A.

Ligase is frequently used the the joining of two stand of DNA . It use in DNA replication at lagging stand and recombination process also.

B.

Fructose 1-6 bisphosphate regelate the activities of the pyruvate kinase with the phenomenon of allosteric regulations.

C

D. To enable selection for only those cells that carry the plasmid with the foreign gene of interest.

In this process many E. coli cell is taken in midium and make recombination in these cells and place in a medium that kill the cell those don't contain the resistance gene and those take resistance gene it means there are recombination because this gene in only in plasmid.

Thank you.

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