5. There are many, many restriction enzymes. Check out NEB, a major supplier of

Question

5. There are many, many restriction enzymes. Check out NEB, a major supplier of restriction enzymes for scientific labs (https:/www.neb.com/products/restriction-endonucleases). Scroll to the bottom of the page and check out the list of enzymes. 0.5 pts each Pick one, Click on the name and write it here: a. What is the recognition site? Show where it cuts both strands with a /(eg. GG/ATCC) b. Does it leave blunt ends or sticky ends (have single strand overhangs)y c.What is the optimal incubation temperature for this enzyme? d. Restriction enzymes are named after the bacterium they were isolated from. For example, EcoRI was isolated from E coli. From what bacterium was your restriction enzyme originally from?

Explanation / Answer

After scrolling to the bottom of the page of NEB- restriction endonuclease, i have chosen the XmaI restriction endonuclease.

a) The recognition site of XmaI is C/CCGGG on one strand and GGGCC/C on the other.

b)It leaves sticky ends and leaves a single strand overhang.

c)The optimal incubation temperature for this enzyme is 37°C.

d)XmaI is taken from Xanthomonas malvacearum

Hope it helps, Good luck !!!!

Please do press like !!!! :)

Related Questions

Navigate

• Browse (All)
• Browse 5
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.