1. Imagine you have engineered a set of genes, each encoding a protein with a pa

Question

1. Imagine you have engineered a set of genes, each encoding a protein with a pair of conflicting signal sequences that specify different compartments. If the genes were expressed in a cell, predict which signal would win out for the following combinations. Explain your reasoning.
(c) Signals for import into mitochondria and retention in ER (ie, the KDEL signal for retrieval from Golgi to ER). (In this case, you are to assume that we have specially engineered the protein to have a KDEL sequence, and a mitochondrial import sequence, but no amino-terminal hydrophobic signal sequence.)

Explanation / Answer

1) It would end up in the ER, since proteins imported into theER are imported co-translationally, while those imported into thenucleus are imported post-translationally. 2) Same as above: the ER. 3) I'm unfamiliar with the KDEL sequence, but try to figure itout by looking at which import signals illicit a co- or post-translational response. Many signals also require more than oneportion to work (for example, an ER stop-transfer sequence is notsufficient to get a protein into the ER. It also needs astart-transfer sequence).

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