QUESTION 3. Curcumin (a molecule found in turmeric) has many health benefits, bu

Question

QUESTION 3. Curcumin (a molecule found in turmeric) has many health benefits, but scientists are still discovering the cellular mechanisms by which it functions. Recently, it was discovered that curcumin targets ATP synthase activity. First, scientists used a biochemical assay to determine that curcumin could physically interact with ATP synthase in vitro. They next wanted to determine whether curcumin could also bind to ATP synthase in cells, and they were able to acquire an antibody that could bind to curcumin to test this. a) What specific type of experiment did they perform in order to visualize in cells) whether it was consistent for curcumin to be binding ATP synthase? (2 pts) b) If curcumin was binding ATP synthase in cells, where would they see the curcumin localization? (Be specific.) (2 pts) c) Why could this experiment not prove that curcumin was binding to ATP synthase in cells? (2 pts) (vocab term is sufficient answer)

Explanation / Answer

a) In order to find out that curcumin was binding to ATP synthase in cells, they must have chosen the specific cell in which they want to see the interaction happening , which in this case will be Mitochondria, as ATPs are made in here. After that when the ATP synthase of the cell was present , curcumin was added and it was hoped that it would go and interct with ATP synthase .Now , to confirm this the antibody that could bind to curcumin should have been made fluoroscently labelled or radio labelled so that when it binds to curcumin , it fluoresces or emits radioactivity.By this, they would have detected the location of curcumin and found out that if it has bound to ATP synthase or not.

b)Curcumin localization will be found in the mitochondrial matrix, because ATP synthase which generates ATP is found in mitochondria.

c)In this experiment as the antibody was binding to curcumin and not the ATP synthase.So the fluoroscence will indicate the binding of antibody to curcumin and not the binding of curcumin to ATP synthase.

Hope it explains your query , Good luck!!!!

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