QUESTION 3 Vibrio cholerae is a bacterium that lives in water and is normally ha

Question

QUESTION 3

Vibrio cholerae is a bacterium that lives in water and is normally harmless to humans. If infected by a certain type of virus it acquires the genes to produce the cholera toxin and now can cause disease in humans. This acquisition of genes is an example of

transfection

transduction

conjugation

binary fission

10 points   

QUESTION 4

How many generations does it take for a single bacterial cell to become 256 bacterial cells? (hint, don't forget to count the first cell)

5

6

7

8

9

10 points   

QUESTION 5

How is DNA replication during conjugation different from DNA replication proceeding binary fission?

DNA replication during conjugation involves only plasmid DNA, DNA replication proceeding binary fission involves only chromosomal DNA

DNA replication during conjugation initially produces a single stranded DNA molecule, DNA replication proceeding binary fission produces double stranded DNA molecules

DNA replication during conjugation involves only a single origin of replication, DNA replication proceeding binary fission?involves many origins of replication

DNA replication during conjugation involves does not require the enzyme DNA polymerase, DNA replication proceeding binary fission requires DNA polymerase

10 points   

QUESTION 6

If you start with one bacterium that can divide every 10 minutes, how many bacteria will there be after one hour?

8

40

64

256

transfection

transduction

conjugation

binary fission

Explanation / Answer

Question 3 - Transduction. - Since only in transduction genes are transferred using viral vectors and insertion occurs specifically.

Question 4- 9. Since 28-256. Xn+1 Here X is bacterial cell and N+1 is number of generations.

Question 5. Answer A. Since in conjugation only plasmid DNA is involved and DNA replication after binary fission involves chromosomal DNA also.

Question 6 - Answer 64. Since P-population = 2(60/10). = 64.

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