Here are some experimental results that apply to the lysosome: Now, which of the

Question

Here are some experimental results that apply to the lysosome:

Now, which of these is evidence that the lysosome is a discrete organelle in the
cytoplasm and is NOT an artifact of the extraction procedure? Choose only one of the
above.
a. Acid hydrolases can be extracted from cells
b. If the extraction is carried out in isotonic media, the enzymes are found in the
pellet of a centrifugation experiment.
c. If the extraction is carried out in hypotonic media, the enzymes are found in
the supernatant of a centrifugation experiment.
d. If cells are fixed and treated with lead nitrate and a proper substrate under
appropriate conditions, and then prepared for electron microscopy, dense vesicles
are seen in the cytoplasm.
e. Lysosomes contain a proton pump.

Explanation / Answer

a

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