Here are some experimental results that apply to the lysosome: i. Acid hydrolase

Question

Here are some experimental results that apply to the lysosome:
i. Acid hydrolases can be extracted from cells
ii. If the extraction is carried out in isotonic media, the enzymes are found in the
pellet of a centrifugation experiment.
iii. If the extraction is carried out in hypotonic media, the enzymes are found in
the supernatant of a centrifugation experiment.
iv. If cells are fixed and treated with lead nitrate and a proper substrate for acid
hydrolyases at a pH of 6, and then prepared for electron microscopy, dense
vesicles are seen in the cytoplasm.
v. Some vesicles contain a proton pump.

Now, which of these results was the best evidence that the enzymes are contained in a
membrane vesicle that existed prior to the extraction?
a. all of them
b. ii and iii
c. iv
d. i and iii
e. ii, iii, and v

Explanation / Answer

a

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