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16. After thorough analysis of the short stretch of DNA below, one will be able

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Question

16. After thorough analysis of the short stretch of DNA below, one will be able to identify embedded within it………… GGTGAGCGATTCGGTAGCCTCCATTCGTTAGCGTAGGCCGCGGCCGGCGCTCGAGCCACACACACACACACACACACACACACACACACACACACTATCGATTTAATTTGAGGTCCGTCGAGTTTAACGCGATTGAGTTGACACCCGGATCGATGTAACGTGGAGAGGGCCATAAATCGGATTACGCCGTTTGAGGTCCGTCGAGTTTAACGCGATTGAGTTGACACCCGGATCGATGTAACGTGGAGAGGGCCATAAATCGGATTACGCCGTTTGAGGTCCGTCGAGTTTAACGCGATTGAGTTGACACCCGGATCGATGTAACGTGGAGAGGGCCATAAATCGGATTACGCCGTTCCATTCTAGCTGCTTGAGGCGGATTAGCTTAGCTCTCTATAGCCCCGCCGCCGCCGCCGCCGCCGCCGCCGGGGGATAGATAGAAAAAAAAAAAAAAA

a. four different microsatellite sequences. b. two minisatellites and four microsatellites c. two minisatellites d. three microsatellites and one minisatellite e. two microsatellites

Explanation / Answer

Microsatellites are repetitive sequences. Individual repeating unit can be 2-13 base pair long. Mostly they are di- 2 , tri-3 or tetra-4 base pair repeats. They are repeated 10-20 times and cluster upto less than 150 base pair.

GGTGAGCGATTCGGTAGCCTCCATTCGTTAGCGTAGGCCGCGGCCGGCGCTCGAGCCACACACACACACACACACACACACACACACACACACACTATCGATTTAATTTGAGGTCCGTCGAGTTTAACGCGATTGAGTTGACACCCGGATCGATGTAACGTGGAGAGGGCCATAAATCGGATTACGCCGTTTGAGGTCCGTCGAGTTTAACGCGATTGAGTTGACACCCGGATCGATGTAACGTGGAGAGGGCCATAAATCGGATTACGCCGTTTGAGGTCCGTCGAGTTTAACGCGATTGAGTTGACACCCGGATCGATGTAACGTGGAGAGGGCCATAAATCGGATTACGCCGTTCCATTCTAGCTGCTTGAGGCGGATTAGCTTAGCTCTCTATAGCCCCGCCGCCGCCGCCGCCGCCGCCGCCGGGGGATAGATAGAAAAAAAAAAAAAAA

In the given sequence there are two microsatellites (underlined) one is a repeat of dinucleotide CA and another is a tri-nucleotide repeat of GCC. There 19 dinucleotide CA repeats and 8 tri-nucleotide GCC repeats

There are no minisatellites.

Each repeating unit is 9-64 base pair long. There are 20-50 repeats and cluster upto 20 kilobase in length.

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