What is the hypotheses for this exercise? experiment LloR With your Ul the with

Question

What is the hypotheses for this exercise? experiment LloR With your Ul the with the entire class. Afterward you will evaluate and be evaluated by your team class-mates. Each student will present their part members on your ability to work together through these experiments. Decid e among your group the following for your control and assigned experiment. Formulate a testable hypothesis Assign roles to the team members . Coordinator-oversee execution of the experiment; keep team members on task; lead team discussions of results Materials manager-gather/return materials; label tubes Experimentalist -will perform the experiment and all group members will help him/her. Recorder-record the findings and plot the graph Materials 1.5 ml microcentrifuge tubes Glucose test strips Student boxes . Test tube rack Glucose strip color scale Floating tube holders (Temperature box only) 5% Sucrose (Specificity box only) 0.5M EDTA (Inhibition box only) Buffer solutions pH 2,4,6,8 & 10 (pH box only) Milk . Lactase . 5% Glucose . Black Sharpie Procedure: Control Experiment -All groups 1. Add 20 drops of 5% glucose to tuba. 2. Add 10 drops of milk and 10 drops of lactase to tube B 3. Add 10 drops of milk and 10 drops of water to tube C 4. Close the lids of all tubes and invert two to three times to mix the solutions. 5. Incubate at 40 oC for 20 minutes in the water bath. 6. Remove the tubes from the water bath. 7. Open tube and insert glucose test strip for one second. egtv we Cow A- dar 8. Get rid of excess fluid on a paper towel. laboratory 4 Enzyme

Explanation / Answer

Hypothesis:

Milk contains lactose, which upon reaction with lactase produces glucose.

Q1: Enzyme: Lactase, Substrate: Lactose, Product: Glucose

Q2: Tubes A and C

Q3: Tube A: Clear colour change to green on strip (Positive control). Tube B: No colour change on strip (Negative control)

Q4: Controls provide a comparison to verify intensity of change. Also controls enable us to ensure that the reaction components are functioning as expected and there are no unexpected variables or contaminants in the reaction.

Q5: Unepected results in the control tube would indicate the presence of contaminants in the reaction components (Milk, lactase or glucose test strip)

Q6: Increasing substrate concentration would yield increasing intensity of green colour of the gucose test strip.

Related Questions

Navigate

• Browse (All)
• Browse W
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.