8. What enzyme methylates A in GATC repeats to regulate ori firing? (7 pts) 9. D

Question

8. What enzyme methylates A in GATC repeats to regulate ori firing? (7 pts) 9. During DNA replication, what protein stabilizes and protects single-stranded DNA? (7 pts) 10. What subunit of the DNA polymerase IIl core h 11. What DNA structure protects the end of the eukaryotic chromosomes? (7 pts) 12. A common type of genetic variation among people, which involves a difference in a single as 3'-5' exonuclease activity? (7 pts) nucleotide, can be used as markers to find disease genes. What is this called? (7 pts) 13. If you are going to test localization of the GAPDH RNA in a tissue, what technology can you use? (7 pts) 14. David planed to test if protein A (molecular weight-25KD) was expressed in the mouse brain. He made mouse brain lysates and ran a western blotting. His result looked like this:

Explanation / Answer

8. Deoxyadenosine methylase is the enzyme which methylates A (adenine) in GATC repeats to regulate ori (origin of replication) firing.

9. The SSB (single-stranded binding) proteins stabilize and protect the single-stranded DNA during DNA replication process.

10. The DNA Polymerase III comprises three subunits: , and subunits. The subunit has the polymerase activity, the subunit has 3'5' exonuclease activity and the subunit stimulates the subunit's proofreading.

11. The DNA structures called telomeres, protect the ends of the eukaryotic chromosomes.

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