8. What is Chargaff\'s rule? 9. You have a vial of double stranded DNA in an aqu
ID: 31071 • Letter: 8
Question
8. What is Chargaff's rule? 9. You have a vial of double stranded DNA in an aqueous solution that is composed 100% of only A -T base pairs. You have a second vial of double stranded DNA in an aqueous solution that is composed 100% of only G -C base pairs. Double stranded DNA can be denatured into single stranded DNA by increasing the temperature. Which vial of DNA will denature at a lower temperature? Why? 10. If you take the two tubes of DNA mentioned in Problem 8, mix them together, denature them both by raising the temperature to 1000, well above the denaturing temperature (melting temperature) of either molecule, and then start SLOWLY lowering the temperature, which molecule will renature first? Will double stranded G -C DNA be formed first, or will double stranded A -T DNA be formed first? 11. Draw and write the full name of the following nucleotides - AMP, GDP, CTP - dGMP, dGDP, dTTP - dCTP, dAMP, UDP, dTMPExplanation / Answer
8)
The nucleotide base pairs A-T and G-C are equal to 1(A+T = G+C = 1). This is known as Chargaaff's rule.
9)
The tu be containing A-T base pairs denatures first tahn the vial containing G-C pairs. This is because, the A-T base pair contain only two hydrogen bonds, whereas, the G-C base pairs contain three hydrogen bonds which are very stable. The two hydrogen bonds can be denatured at high temperature easily than the three hydrogen bonds. Hence, the vial containing A-T base pairs denatures first.
10)
The DNA with A - T base pairs reanneals first as it require only two hydrogen bonds rather than 3 hydrogen bonds between G-C base pairs.
11)
Adenosine monophosphate, guanosine diphosphate and cytidine triphosphate
Deoxyguanosine monophosphate, deoxyguanosine diphosphate and Deoxythyminetriphosphate.
Deoxycytidine triphosphate, Deoxyadenosine monophosphate, Uridinediphosphate and deoxythymidine monophosphate.
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