Yeast GUF Project: Module 1 Worksheet BASIC INFORMATION Assigned Gene Systematic

Question

Yeast GUF Project: Module 1 Worksheet BASIC INFORMATION Assigned Gene Systematic Name YKL142W SGD Description of Known Information about your Protein Protein of unknown function; undergoes transcription induced under cell wall stress: protein levels are reduced under anaerobic conditions rotein abundance increases in response to DNA replication stress: originally thought to be a mitochondrial ribosomal protein based on sequence analysis DNA Coordinates Chromosome #: Gene Size Calculation: 178515-179174 660 DNA Sequence: Protein length (aa 219 Does this length make sense with your calculated DNA sequence length? Why or Why not? 25081.3 Molecular Weight (Da Isoelectric Point Protein Sequence: ATGTCTAACG AAATTGAATT ACTGCAGAAG CAAGTCTCTG AATTACAAGA CTTAGTGAAG 61 AAGCAAAGTC TGATTATCTC TAAGACAGGA GAGCGTGTGC TGGAATTGCA GTTAGATAAA 121 CAAAAGCACG ACGTAACTGA TTTTGATTCG AAATTCTCTA AATCTATCTC GAAGAAATCA 181 GGATCCGCTA CTCAATTCGA TGCCACTGAT TTTGCCACAA ATGAAGATCT AGTGGAATTA 241 GTCAAAGAAC TACAGGGTGA GCTAAATTTC ATCGAGGAAA GGTCTATCAG AAGACTTGTT 301 AACTCTTTGA AAAAAGATGA TGATGACGTT ATAGCGCCAC TGCCTAATGC AGATGGTGAT 361 ATTCCTGCTA TTTCAGACGG AGTGTTCCCT AAGTCGTTGA AAGAATTTAA AGACATCCCA 421 GACTTAAAAC TGGTAAGACT TGCTAAATTC TACGAAAGAT TGCCACCAAC TCTAAAGGAA 481 CAAGAAGATT TTGAAAACTT CCTGGAAGGT AAGGTGGAAG CCTTTCACAT TAATGAGACC 541 ACTGATGAAG AGATTTCTAA GGAGTTGGAA AAGTTCTCCA AAGATGAGCT AGATGATGCA 601 TTTAATGATG TTGCCCGCTA TCTAGGTCTC TCCCTTAGAA GAGGAACCGA AATATGGTAA

Explanation / Answer

All the filled answers are correctly done and hence, require no change.

But gene sequence is written in place of protein sequence, so it should be corrected.

There is only one question to be answered.

Does this length make sense with calculated DNA sequence?

Yes, it surely and perfectly does.

DNA sequence has 660 nucleotides, these code for 220 codons, out of which 219 get translated as amino acids whereas the last codon was the chain termination codon which is not seen here.

Hence, it makes perfect sense.

Only 1 question was to be answered.

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