Yeast GUF Proiect: Module 1 Worksheet BASIC INFORMATION Assigned Gene Systematic

Question

Yeast GUF Proiect: Module 1 Worksheet BASIC INFORMATION Assigned Gene Systematic Name YKL142W SGD Description of Known Information about your Protein _ Protein of unknown function; undergoes transcription induced under cell wall stress: protein levels are reduced under anaerobic conditions rotein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis DNA Coordinates: Chromosome #: Gene Size Calculation: 178515-179174 LJ(11) 660 DNA SeCG AAATTGAATT ACTGCACGTGTGC TGGGAAGAAATCA 1 ATGTCTAACG AAATTGAATT ACTGCAGAAG CAAGTCTCTG AATTACAAGA CTTAGTGAAG 61 AAGCAAAGTC TGATTATCTC TAAGACAGGA GAGCGTGTGC TGGAATTGCA GTTAGATAAA 121 CAAAAGCACG ACGTAACTGA TTTTGATTCG AAATTCTCTA AATCTATCTC GAAGAAATCA 181 GGATCCGCTA CTCAATTCGA TGCCACTGAT TTTGCCACAA ATGAAGATCT AGTGGAATTA 241 GTCAAAGAAC TACAGGGTGA GCTAAATTTC ATCGAGGAAA GGTCTATCAG AAGACTTGTT 301 AACTCTTTGA AAAAAGATGA TGATGACGTT ATAGCGCCAC TGCCTAATGC AGATGGTGAT 361 ATTCCTGCTA TTTCAGACGG AGTGTTCCCT AAGTCGTTGA AAGAATTTAA AGACATCCCA 421 GACTTAAAAC TGGTAAGACT TGCTAAATTC TACGAAAGAT TGCCACCAAC TCTAAAGGAA 481 CAAGAAGATT TTGAAAACTT CCTGGAAGGT AAGGTGGAAG CCTTTCACAT TAATGAGACC 541 ACTGATGAAG AGATTTCTAA GGAGTTGGAA AAGTTCTCCA AAGATGAGCT AGATGATGCA 601 TTTAATGATG TTGCCCGCTA TCTAGGTCTC TCCCTTAGAA GAGGAACCGA AATATGGTAA Protein length (aa 219 Does this length make sense with your calculated DNA sequence length? Why or Why not? Molecular Weight (Da 25081.3 Isoelectric Point 4.4

Explanation / Answer

Answer- No, the protein length i.e. 219 aa does make sense with the calculated DNA sequence length.

Calculation-

219 amino acid weight will be-

219 X 110 (one amino acid weight)= 24090dalton or 24.09kDa (molecular weight also vary)

now-

Number of bases in 24.09kDa will be-

24.09 x 103 /110 = 219 bases

and if 219 nucleotide bases are there means 657bp, i.e. it is not matching with 660bp

Thanking You

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.