You have a matrix protein with an isoelectric point of 9, and you develop a proc

Question

You have a matrix protein with an isoelectric point of 9, and you develop a process for creating microspheres out of this material. If you loaded the compound with B-lactoglobulin, myoglobin, and lysozyme what would the anticipated order be for the release of the compounds? I.E. Which one would be released, first, second, third etc. Why do you anticipate this order of release? How would you need to modify the matrix in order to reverse the order of release? What would you need to do to make it so that they all released at approximately the same time?

Explanation / Answer

The isoelectric point of any molecule represents the pH at which the molecule possess no net charge. The molecule is electrically neutral at its particular isoelectric point.

If one wants to create the microspheres from the matrix protein having the isoelectric point of value 9 by loading the compounds sucg as B-lactoglobuli, myoglobulin, and the lysozyme, the order of the release of the compound would be lysozyme first, then the myoglobulin, and lastly the Beta-lactoglobulin.

The rate of realease of the proteins depends on the elctrostatic nature and size of the protein.The size of the myoglobin is smaller as compared to the other two proteins , hence it would release at a faster rate. The order of release is anticipated for knowing the various range of mobilities of the protein and for determining the diffudion coffiecients of various types of the proteins.

In order for reversing the oreder of release of the proteins, the pore size and gelation strength of the matrix should be changed according to the charge and size of the differnt types of the proteins. The matrix should be made electrically neutral in order to get the realease of the proteins at the same time.

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