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Question 7 Not yet answered Marked out of 1.00 P Flag question Which of these me

ID: 150749 • Letter: Q

Question

Question 7 Not yet answered Marked out of 1.00 P Flag question Which of these methods can be used in combination with PCR to study the binding of a protein to a DNA sequence of interest? Select one: a. there is no way to do that, proteins can't bind to DNA b. RNA immunofluoescence (RIF) assay c. chromatin immunofluoescence (ChlF) assay d. RNA immunoprecipitation (RIP) assay e. chromatin immunoprecipitation(ChlP) assay Question Not yet answered Marked out of 1.00 P Flag question How do the 3' ends of canonical histone H3.1 and the histone H3.3 variant mRNAs differ? Select one: O a. histone H3.1 mRNAs are not polyadenylated but they have 3' hairpin structure instead b. there is no difference in the organization of these mRNAs O c. only variant histone H3.3 genes have introns d. none of these answers is correct e. only canonical histone H3 genes have introns

Explanation / Answer

answer 7-

E- chromatin immunoprecipitation assay(ChIP).

it is used to determine whether specific proteins are associated with specific genome region or not.. ChIP is used to study DNA-protein interaction .

answer 8-

A -- histone H3.1 mRNA are not polyadenylated but they have 3' hairpin structure instead.

H3.1 histone is synthesized during S phase of cell cycle only, and it does not have poly A tail in its mRNA. while H3.3 histone can be synthesized at any stage as they have poly A tail.

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