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Suppose a researcher uses a single SELEX experiment to select for an RNA molecul

ID: 150763 • Letter: S

Question

Suppose a researcher uses a single SELEX experiment to select for an RNA molecule that catalyzes the hydrolysis of a particular ester. Select statements that describe good design parameters for her SELEX experiment. **NEED answers in few hours**

Suppose a researcher uses a single SELEX experiment to select for an RNA molecule that catalyzes the hydrolysis of a particular ester. Select statements that describe good design parameters for her SELEX experiment. The chromatographic resin must be bound to an analog of the reactants of the ester hydrolysis reaction. The practical limit for length of her RNA molecule is 10 nucleotides. A transition-state analog of the ester hydrolysis reaction will be tethered to a chromatographic resin. A 30 nucleotide RNA is beyond the practical size limit of her SELEX experiment. RNAs with potential hydrolysis activity will be captured by ion exchange chromatography.

Explanation / Answer

RNA with potential hydrolysis activity will be captured by ion exchange chromatography describes the good design parameter for the SELEX experiment

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