n E. coli bacterium that is partially diploid for the lac operon and has the fol
ID: 151091 • Letter: N
Question
n E. coli bacterium that is partially diploid for the lac operon and has the following genotype: lacl is the sequences coding for the lac repressor protein, lacP is the promoter, lacO is the operator, lacz is the sequence coding for beta galactosidase, and lacY is the sequence coding for permease. The lacoc mutant (the C stands for "constitutively active') has a "irrepressible" mutant operator sequence. The'+" superscript indicates a gene that produces protein with normal function, but a'- superscript indicates the resulting protein is nonfunctional. Under what lactose concentrations would you expect to find enzymatically active (functional) beta galactosidase and permease? Briefly explain.Explanation / Answer
Answer:
The galactosidase will synthesize all the time constitutively whereas the expression of permease requires the presence of lactose.
Explanation:
Although, one repressor coding gene (lacI-) is non-functional but repressor coded by another gene (lacI+) is enough to do whole functioning.
The one operator is mutated (lacOC) so it cannot be repressed by the repressor and will transcribe galactosidase constitutively but this segment also has mutated lacY- so it can’t make permease and require another gene.
Although, one galactosidase gene (lacZ-) is non-functional but another functional gene (lacZ+) is enough to do whole functioning.
Similarly, one permease gene (lacY-) is non-functional but repressor coded by another gene (lacZ+) is enough to do whole functioning.
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