This is some of the questions Name: Pre-lab Homework for BIOL 150 for TPSS Campu
ID: 151191 • Letter: T
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This is some of the questions
Name: Pre-lab Homework for BIOL 150 for TPSS Campus-Enzymes (Exercise 4B) Read Exercise 4B: Enzymes in your lab manual and then complete the following questions 1. Define enzymes? What type of macromolecule are they? 2. Why are enzymes important for catalyzing chemical reactions? 3. What is the relationship betwcen enzgyme activity and its 3D structure? What bonds are important for this 3D structure? 4. Define a substrate. Explain how the substrate and active site of the enzyme are related to each other 5. What happens to the enzyme when it is not close to optimal conditions? What happens to the structure of the enzyme? 6. Compare the shape of the enzyme when it is at the optimal temperature to when it is a Compare the shape of the enzyme when it is at the optimal pH to when it is a far from optimal pH. 7.Explanation / Answer
1.Enzymes are defined as biocatalysts synthesized by living cells, they are protein in nature (except -RNA acting as riboenzyme), colloidal and thermoalabile in character, and specific in their actions.
*Enzymes are in high molecular weight so it is a macromolecule, as it is made up of either protein or RNA(riboenzyme) ,so that it helps in speed up chemical reaction in biological system.
2.Enzymes are important for catalysing chemical reaction because ,they are also similar to other chemical catalysts in many ways
*enzyme can affect the rate but not equlibrium constant of a chemical reaction.
*Enzymes increase the rate of chemical reaction in both directions, forward and reverse.
*Enzymes bind their substrate not permanently, but transiently for a time. The portion of an enzyme that binds substrate and carries out the actual catalysis is termed the active site.
3. The 3D structure of an enzyme that determines its activities, enzymes molecules which are protein folded to various shape, changing the order of amino acid would cause it to fold into a different shape and substrate molecules would no longer fit into the active site,
*ionic bonds, covalent bond and hydrogen bond are important for 3D structure.
4.A substrate is a molecule that acted upon enzyme, loaded into the active site of enzyme or the place that allows weak bonds to be formed between two molecules.
*The functional location on the enzyme, where catalysis occurs, that is called active site, substrate is the molecule the enzyme works on, the substrate bind to enzyme active site.
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