13) Which of the following would elute last from a size-exclusion chromatography
ID: 151282 • Letter: 1
Question
13) Which of the following would elute last from a size-exclusion chromatography column a) b) c) d) Protein A: MW-108,000, net charge +2 Protein B: MW-38,000, net charge +1 Protein C: MW-62,000, net charge-+4 Protein D: MW-84,000, net charge-0 14) True or false: not all cells have a plasma membrane. 15) How can cells prevent osmotic effects from causing them to burst? 16) By convention, the sequence of amino acids in a peptide or protein is written from the_ terminus to theterminus. 17) If the K, of a weak acid is 0.000356, what is its pk,?Explanation / Answer
13. b. Protein B: MW= 38,000, net charge= +1
In size exclusion chromatography, separation of molecules is based on their size and in many cases, molecular weight. In this technique, small molecules having less molecular weight are trapped into porous beads and take greater time to elute as compared to larger molecules having higher molecular weights.
In the given question, protein B has the least molecular weight. So, it will elute last.
14. False.
Plasma membrane is essential for survival for a cell even if it lacks cell wall. Without a membrane, a cell cannot survive.
16. By convention, the sequence of amino acids in a peptide or a protein is written from the N - terminus to the C - terminus.
N - terminus refers to the end where the free NH3+ group is located and C - terminus refers to the end, where the free COO- group is located.
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