Gene Regulation in Prokaryotes- Lac Operon ECOLIMUTATIONS Use the figure below d
ID: 151727 • Letter: G
Question
Gene Regulation in Prokaryotes- Lac Operon ECOLIMUTATIONS Use the figure below depicting a wild type operon (a) and examples of mutation (b-d) to answer the following questions. c) repressor mutation) (a) I' (wild type) Repressor protein mutation prevents repressor binding Repressor binds operator to the operator and and forms an inducer- epressor complex when nducer is presen produces consitutive synthesis of the lac operon lac repressor protein Allolactose Mutant protein ld) P(super-repressor mutation (b) (operator constitutive mutation) Opeatorsite mutation prevents repressor proten binding and leads to constitutive synthesis of the ac operon Repressor protein mutation blocks binding to the inducer preventing formation of the nducer-repressor complex Mutant nepressor proten binds to the operator preventing tanscription lac repressor mutant 1) (2 pts) You are studying lactose metabolism and have been culturing E colí in different medias. One media has only glucose, one has only lactose, and a third has both glucose and lactose (1 pt) You notice that some of the cultures grown in the presence of both glucose and lactose are metabolizing lactose. Which type(s) of mutation(s) could cause this to occur? a. (1 pt) You notice that some E. colí cultures failed to grow in the presence of only lactose. You know that normal E. coll are able to metabolize lactose so something must be wrong. Which mutation(s) could explain this? b. (2 pts) Why is it advantageous for organisms to be able to regulate their gene expression? Please explain in 1-3 sentences. 2)Explanation / Answer
Q1 (a): OC and I- mutation as these are constitutive mutations.
Q1 (b): IS mutation as repression always remain bind to operator and transcription never occur.
Q2: By having control on gene expression, organism can choose when to express a gene and when not. This will help in saving energy and cellular resources. There is no use of transcribing a protein indefinitely if its not going to use.
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