Gene Regulation in Prokaryotes-Lac Operon (4 pts) Describe what is happening in
ID: 198561 • Letter: G
Question
Gene Regulation in Prokaryotes-Lac Operon (4 pts) Describe what is happening in the following scenario to the repressor, operator, cAMP production, CAP, CAP binding site, RNA polymerase, and whether or not mRNA gets translated. Be sure to state what effects Lactose and Glucose are having in the appropriate space. 1. Glucose absent, lactose absent: lacZ acY acA lacl CAP P O 3" Repressor & Operator (1 pts): cAMP production and CAP (1 pts): CAP binding site & RNA polymerase (1 pts): mRNA (1):Explanation / Answer
Given condition: No lactose and no glucose.
When lactose is absent, the repressor is functional. It binds to the operator.
Since the operator is bound by the repressor, RNA polymerase can not bind to the promoter.
Since RNA polymerase can not bind to the promoter, transcription can not take place. So, mRNA will not be produced.
When glucose is absent, the cAMP-CAP complex can bind to the upstream region. But, RNA pol can not be recruited as the repressor is already present.
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