Consider the following data obtained from L1210 tumor cells in answering questio

Question

Consider the following data obtained from L1210 tumor cells in answering questions 19 21: ring the purification of the enzyme dihydrofolate reductase Crude Extract nlmg/ml Enzyme Activity,units/ml 3000 0.100 Affinity Column 1.50 20.0 100.0 19. What was the percent recovery of the purification process? a) 0.05 b) 0.10 c) 0.50 d) 20 e) 50 20. What was the specific activity of the final enzyme preparation? a) 2.0 b) 5.0 c) 30 d) 100 e) 200 21. What was the purification-fold for the purification process? a) 10 b) 100 c) 200 d) 1000 e2000 22. Treatment of the ß-chain of insulin with Sangers reagent followed by acid hydrolysis yields a DNP Phe. Partial hydrolysis of the B-chain of insulin with pepsin followed by separation and Edmann sequencing of the peptides formed yields the sequences below: QHLCGSHL EALYLVC RGFFYTPKA FVNQHL GSHLVEALY LVCGERGF Which of the following is the c-terminal amino acid of the B-chain of insulin? c) F e) imidazole 23. What type of ring is present in the side-chain of histidine? d) purine c) guanidinium b) pyrimidine a) indole

Explanation / Answer

23) what type of ring present in side chain of histidine - Imidazole

Imidazole exists in 2 tautomeric forms with hydrogen atom moving between the two nitrogens.

2 nitrogen atoms: one is basic and the other is not.

Related Questions

Navigate

• Browse (All)
• Browse C
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.