Enzymes TABLE 3 Contonts of the Throe Experimental Tubes Tube Disnille Poteto Di
ID: 152255 • Letter: E
Question
Enzymes TABLE 3 Contonts of the Throe Experimental Tubes Tube Disnille Poteto Distililed Extract 0.5 ml 0.5 ml 0.5 m 0.5 ml PTU 5 mL 5 ml 5 ml 0.5 mL 0.5ml 0.5 ml 1 ml 1 mL 0.5 ml 1. Using Table 3, prepare three experimental tubes. Be solutions in the sequence given in the table (water first, potato extract next, PTU next, etc.). Cover each tube with a fresh Parafilm square and mix Which test tube is the control? sure to add 2. 3. Why was the concentration of catechol increased in test tube 2? 4. Why should the catechol be added to the test tubes last? 5. Record your observations in the Results section, and explain your results in the Discussion section. 6. After recording your results in Table 4, dispose of the solutions in the test tubes in the waste container indicated by your instructor. Do not pour down the drain. Results Design a table to record your results (Table 4) in the margin. Discussion 1. Explain your results in terms of your hypothesis TABL ExpeExplanation / Answer
2. Test tube number 3 is control as it is not containing any PTU extract, while other two tubes contain PTU extract with different volumes.
3. The volume of catechol in tube nuber 2 is high, it is because to assess the enzyme activity of catechol oxidase on two different initial substrate concentration. The final volume or total volume in all three tubes should be 7ml.
4. The PTU or potato tuber extracts contain catechol oxidase, this is an enzyme which acts on the catechol. Hence to assay the enzyme activity of catechol oxidase, catechol is used as substrate in this experiment.
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