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Several mutants are isolated, all of which require compound G for growth. Interm

ID: 15325 • Letter: S

Question

Several mutants are isolated, all of which require compound G for growth. Intermediate compounds in the biosynthetic pathway to G (A to E) are known but their order in the pathway is not. Each of five mutants are tested for their ability to grow on each of the intermediates with the results shown in the table below. "+" = growth, "-" = no growth.
Intermediate tested A B C D E G
Mutant 1 - - - + - +
Mutant 2 - + - + - +
Mutant 3 - - - - - +
Mutant 4 - + + + - +
Mutant 5 + + + + - +


4. [2pt]
What is the order of the compounds A through E in the biochemical pathway? Enter your answer as a string of six letters starting with the earliest intermediate in the pathway (e.g. ABCDEG if A-B-C-D-E-G).
Answer: EACBDG

5. [2pt]
At which step in the pathway is each mutant blocked? Give your answer in the order of the mutant numbers, so if mutant 1 is blocked at A-B and mutant 2 is blocked at B-C etc., enter AB,BC,CD,DE,EG.
Answer: ?

Explanation / Answer

5. BD,CB,DG,AC,EA

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