Attached is question 3 for my biochem exam guide. Question #3. A) The spider ven

Question

Attached is question 3 for my biochem exam guide.

Question #3. A) The spider venom from the Chilean Rose Tarantula Grammostola patulata contains a toxin that is a 34-amino acid protein. It is thought to be a globular protein that partitions into the lipid bilayer membrane to exert its effect. The sequence of the protein is: ECGKFMWK CKNSNDC CKDLVCSSR WAKWCVLASPF 1) Identify the hydrophobic and highly hydrophilic residues in the protein. 2) The protein is thought to have a hydrophobic face that interacts with the lipid membrane. How can the hydrophobic amino acids far apart in sequence interact to form a hydrophobic face? B) You are studying the effects of amino acid replacements on the stability of a particular o-helix that is buried in the protein myoglobin. You carry out a series ofreplacements of a particular leucine reside located in the helix, using site- specific mutagenesis. The replacements are as follows: alanine to histidine i) alanine to phenylalanine ii) isoleucine to proline iv) isoleucine to arginine v) leucine to isoleucine 1)For each replacement, predict whether the change would destabilize, stabilize or have no effect on the structure of the o-helix. Briefly explain your predictions. 2) For each replacement, write the most likely mRNA codon required to code for the particular amino acid. Which of the replacements can be carried out by a single-base change? Which ofthe replacements can be produced only by altering two bases in the mRNA codon?

Explanation / Answer

A 1

Hydrophilic- C,W

Hydrophobic- A,L, F, V,P,G

2

The insoluble hydrophobic aa cannot be pushed out as they are attached to the hydrophilic. All they can do is group together forming a droplet.

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