A special class of ABC-type ATPases associated with tumors that allow movement o

Question

A special class of ABC-type ATPases associated with tumors that allow movement of antibiotics and other drug out of the cell are known as human catalytic convertase. import transporter proteins, multidrug resistance transport protein. cystic fibrosis transmembrane conductance regulator. none of the above; no such transporter has been identified in eukaryotes Where would you expect to find an organism possessing a cell membrane composed of large proportions of cholesterol and a predominance of unsaturated fatty acids? upper levels of an aquatic environment temperate regions tropical regions equatorial regions frozen wastelands Base your answers to the questions on the following information: For a particular cell, the concentration of sucrose is 10 mM on the inside of the cell and 0.5 mM on the outside, whereas the concentration of sodium ions (Na^+) is 0.5 mM on the inside of the cell and 10 mM on the outside. The membrane potential is -150mV, and the temperature is 25 degree C. Constants: R = 1.987 cal/mol degree; T = 25 degree C = 298 K; F = 23, 062 cal/mole volt What is the delta G' for the inward transport of sucrose? -1.774 kcal/mol; an endergonic reaction -0.774 kcal/mol; an exergonic reaction +1.774 kcal/mol; an exergonic reaction +0.774 kcal/mol; an endergonic reaction none of the above What is the delta G' for the inward transport of sodium ions? +5.233 kcal/mol; an endergonic reaction -5.233 kcal/mol; an exergonic reaction +0.233 kcal/mol; an exergonic reaction -0.233 kcal/mol; an endergonic reaction none of the above What would be the net delta G' for the coupling of these two reactions? -7.007 kcal/mol +7.007 kcal/mol +1.774 kcal/mol -1.774 kcal/mol none of the above Base your answers to the questions below on the following information: A cell biologist interested in the transport of calcium ions Ca^2+ across the plasma membrane of bacterial cells made the following measurements on bacterial cells at 25 degree C: Vm = -150 mV [Ca^2+ inside = 20 mM [Ca^2+ outside = 0.4 mM What is the free energy change for the inward movement of calcium ions into the bacterial cell? -3.6 kcal/mol; an exergonic reaction -3.6 kcal/mol; an endergonic reaction +3.6 kcal/mol; an exergonic reaction +3.6 kcal/mol; an endergonic reaction

Explanation / Answer

1. G = RT ln [A in / A out]

G = (1.987) × (298) x ln (10/0.5)

       =1773.85 Cal/mole

        =1.774 kcal/mole

Thus the correct answer is option (C).

2. G = RT ln [A in / A out] + (z)(F) (Vm)

       = (1.987) × (298) x ln (0.5/10) + {1 x 23.062 x (-150)

       = -5233.15 Cal/mole

       = -5.233 kcal/mole

Thus the correct answer is option (B)

3. NET G = -1.774 -5.233

             = -7.007 option (A)

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